Capillary electrophoretic analysis of hydroxyl radicals produced by respiring mitochondria
نویسندگان
چکیده
منابع مشابه
Apolipoprotein E genotyping by capillary electrophoretic analysis of restriction fragments.
We present the genotyping of apolipoprotein (apo) E by means of restriction fragment analysis of amplified genomic DNA by high-performance capillary electrophoresis and a replaceable non-gel-sieving matrix. This procedure streamlines the genotyping of apo E in large-scale population studies because of the automation and speed of capillary electrophoresis.
متن کاملAdenine oxidation by pyrite-generated hydroxyl radicals
Cellular exposure to particulate matter with concomitant formation of reactive oxygen species (ROS) and oxidization of biomolecules may lead to negative health outcomes. Evaluating the particle-induced formation of ROS and the oxidation products from reaction of ROS with biomolecules is useful for gaining a mechanistic understanding of particle-induced oxidative stress. Aqueous suspensions of p...
متن کاملElectrophoretic Analysis of Colored Product Produced by Schiff-formaldehyde Reaction
According to Wieland and Scheuing (1), the Schiff-aldehyde reaction proceeds in two steps. In the first step, the fuchsin-sulfurous acid reagent links with 1 mole of aldehyde to form a monoaldehydic compound, and in the second step, the compound again links with SO2 and another mole of aldehyde to form a dialdehydic one. The present author has unexpectedly found that the colored product produce...
متن کاملImprovement of Capillary Electrophoretic Enantioseparation of Fluoxetine by a Cationic Additive
One of the problems encountered in CE separations of basic compounds is the adsorption of analytes onto the negatively charged capillary wall which could lead to poor repeatability of migration time and peak area. Additionally, separation of enantiomers of chiral of basic drugs is commonly carried out in low pH buffer which contributes to strong ionic interaction of the cationic drug ions with ...
متن کاملCapillary electrophoretic techniques toward the metabolome analysis*
Metabolome analysis is a systematic chemical analysis of metabolites, which may be used to investigate the metabolic activity in the cell. Capillary electrophoresis (CE) is one of the most promising techniques for the metabolome analysis, because it gives high-resolution separations in a reasonable time and requires a minimum amount of samples. General characteristics of CE are discussed from t...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Analytical and Bioanalytical Chemistry
سال: 2013
ISSN: 1618-2642,1618-2650
DOI: 10.1007/s00216-013-7022-y